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Recipes

Chemicals used for the preparation of solutions and media:

  • EDTA used for 0.5M EDTA; 50x TAE; 10x TBE
  • Tris used for 1M Tris-HCl pH 8.8; 8.0; 7.5; 50x TAE; 10x TBS
  • NaCl used for 5M NaCl; LB (low salt); LB (high salt); 10x TBS; 10x PBS
  • sucrose used for MS media
  • boric acid used for 10x TBE
  • tryptone used for LB
  • yeast extract used for LB
  • LB low salt used for LB
  • LB high salt used for LB
  • MS with vitamins used for MS
  • plant agar used for MS
  • micro agar used for LB
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